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KMID : 1031120140040010007
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Journal of Epilepsy Research
2014 Volume.4 No. 1 p.7 ~ p.13
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Effect of Androsterone after Pilocarpine-induced Status Epilepticus in Mice
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Cho In-Ja
Cho Yang-Je
Kim Hyun-Woo
Heo Kyung
Lee Byung-In
Kim Won-Joo
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Abstract
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Background and Purpose: Neurosteroids exert their antiepileptic effects via GABAA and NMDA receptors. Another cell death mechanism is excessive Ca2+ influx into cells. Calbindin-D28k (CB) is a protein that modulates intracellular Ca2+ in the nervous system. We evaluated whether androsterone up-regulates the expression of CB and has a neuroprotective effect by controlling Ca2+ after pilocarpine-induced status epilepticus (SE) in mice.
Methods: SE was induced in ICR mice by injection of pilocarpine. Two hours after SE, mice were treated intraperitoneally (i.p.) with androsterone (100?200 mg/kg) or vehicle, and compared with other control groups. Two days after injection, immunohistochemical staining for CB was performed using a hippocampal slice from each mice group. We also used cresyl violet staining to compare changes in hippocampal structures.
Results: Two days after pilocarpine-induced SE, androsterone increased the expression of CB in the hippocampus compared with control SE mice. The number of CB-positive cells was 1±0.4 cells/mm3 in pilocarpine-only group, 14±1.1 cells/mm3 in pilocarpine plus androsterone 100 mg group and 29±2.5 cells/mm3 in pilocarpine plus androsterone 200 mg group (p<0.001).
Conclusions: These results suggest that the neuroprotective effect of androsterone after pilocarpine- induced SE may be mediated by an increased expression of CB.
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KEYWORD
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Neurosteroid, Androsterone, Calbindin-D28k, Hippocampus, Seizure
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